Number eight Sarah Hunter and flanker Marlie Packer will co-captain England as they begin the defence of their Women’s Six Nations title this month.
Scrum-half Natasha Hunt and number eight Sarah Beckett return after being left out of England’s World Cup squad.
Pregnant lock Abbie Ward and regular centre and vice-captain Emily Scarratt, who is injured, are unavailable for selection but will remain with the group and assist the coaching set-up.
“There are a number of new faces in this squad and while we are missing experienced players, this is a great opportunity for these younger players and we’re looking forward to working with them,” said Middleton.
“Sarah has made it clear she will not be involved in the 2025 World Cup as a player, so it’s essential we look to continue to grow our leadership quality and depth.
Middleton became head coach in 2015 and has led England to five Six Nations titles and four Grand Slams.
He added: “Both Abbie Ward and Emily Scarratt expressed a wish to support our Six Nations campaign despite their playing absence.
“Coaching is an area of the women’s game we all recognise as one of the next big pillars to put in place, so it stands to reason that we jump on this opportunity to support both in an area they have expressed a wish to develop.”